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DNA sequence changes of mutations in the histidine operon control region that decrease attenuation.

W M Barnes, E Tuley

    Journal of Molecular Biology
    |April 15, 1983
    PubMed
    Summary
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    This study details 18 DNA mutations in Salmonella typhimurium

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • The histidine operon in Salmonella typhimurium is regulated by transcriptional attenuation.
    • Attentuation involves the formation of alternative RNA stem-loop structures in the leader sequence.
    • These structures control transcription termination based on histidine availability.

    Purpose of the Study:

    • To characterize DNA sequence changes in the histidine operon control region.
    • To investigate the effects of these mutations on gene expression.
    • To provide evidence for the RNA stem-loop model of attenuator regulation.

    Main Methods:

    • Isolation and characterization of His+ pseudorevertant mutations.
    • DNA sequencing of mutations in the histidine operon control region.

    Related Experiment Videos

  • Analysis of mutation effects on RNA secondary structures.

    • Main Results:

    • Eighteen DNA mutations were identified in the histidine operon control region.
    • Most mutations increased operon expression by affecting transcription termination.
    • Mutations altered RNA stem structures, supporting the competing stem model of regulation.
    • Two mutations suggested ribosome interaction with RNA structures.

    Conclusions:

    • The findings support the prevailing model of attenuator regulation in the histidine operon.
    • Alternative RNA stem structures are crucial for controlling gene expression.
    • Ribosome dynamics may also influence RNA structure-based regulation.