Radiometric assay for thiamine pyrophosphokinase activity in human leukocytes

The activity of thiamine pyrophosphokinase (EC 2.7.6.2) has been demonstrated in the extract from human leukocytes using radiometric assay method. It was estimated to be 17.2 +/- 6.6 p moles of thiamine pyrophosphate formed per mg protein per hour at 37 degrees C. No thiamine pyrophosphokinase activity was detected in hemolysate of human erythrocytes under the assay condition employed.