The detection of specific [3H]imipramine binding in bovine retina

Using [3H]imipramine, specific imipramine binding was demonstrable in the crude membrane homogenate of bovine retina. Scatchard analysis of the saturation experiments revealed an apparent dissociation constant (Kd) of 6.4 nM and a maximal number of binding sites of 780 fmol/mg protein. These results and the substrate specificity show that the properties of imipramine binding to retinal membranes are essentially the same as described for the rat brain.