Expression of interferon-alpha and interferon-beta genes in human lymphoblastoid (Namalwa) cells

Treatment of human lymphoblastoid (Namalwa) cells with Sendai virus induced the coordinate synthesis of both IFN-alpha and IFN-beta interferon mRNAs. One sub-line of Namalwa cells (WRL) produced no IFN-beta activity, although IFN-beta mRNA was induced and was associated with polysomes. The IFN-alpha mRNA was heterogeneous, ranging in size over 1.20-1.35 X 10(3) bases, probably because of variation in the size of the transcribale DNA in the alpha-gene family. The IFN-beta mRNA was monodisperse with a size of 1.05 X 10(3) bases. The kinetics of accumulation and decay of both IFN-alpha and IFN-beta mRNAs, as assessed by hybridization with cDNA probes, were very similar to those of translatable interferon mRNA, as assessed by translation in Xenopus oocytes. Treatment of the cells with butyrate or 5'-bromodeoxyuridine increased the amount of hybridizable IFN-alpha and IFN-beta mRNA about 15-fold and 4-fold respectively, again demonstrating coordinate control of IFN-alpha and IFN-beta production.