Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Simultaneous reversion for genes thy, dra and drm in Bacillus thuringiensis].

T M Grigor'eva, R R Azizbekian

    Genetika
    |March 1, 1983
    PubMed
    Summary

    Mutations in Bacillus thuringiensis genes dra and drm were studied. Simultaneous reversions for thymine requirement and thymidine sensitivity (Thy + Tdr) were observed, linked to rough colony morphology.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Electron microscopy of the surfaces of bacillary spores].

    Mikrobiologiia·2014
    Same author

    [The duration of action of Bacillus thuringiensis spp. israelensis and Bacillus sphaericus after encapsulation by infusoria Tetrahymena pyriformis].

    Meditsinskaia parazitologiia i parazitarnye bolezni·2003
    Same author

    [Isolation, biological properties, and spatial structure of an antibiotic loloatin A].

    Bioorganicheskaia khimiia·2002
    Same author

    [Use of the recombinant bacterial strain to control blood-sucking mosquito larvae].

    Meditsinskaia parazitologiia i parazitarnye bolezni·2001
    Same author

    [Duration of the larvicidal effect of spore crystalline mass of bacteria Bacillus thuringiensis spp. israelensis and Bacillus sphaericus in the laboratory setting].

    Meditsinskaia parazitologiia i parazitarnye bolezni·2001
    Same author

    [Enhancement of the efficacy of Bacillus sphaericus during its encapsulation by Tetrahymena pyriformis infusorians].

    Meditsinskaia parazitologiia i parazitarnye bolezni·1997

    Area of Science:

    • Microbial genetics
    • Bacterial mutation studies
    • Bacillus thuringiensis research

    Context:

    • Investigating genetic mutations in Bacillus thuringiensis var. galleriae 351.
    • Focus on mutations in thy, deoxyriboaldolase (dra), and phosphodeoxyribomutase (drm) genes.
    • Dra and drm mutants exhibit sensitivity to thymidine (Tds).

    Purpose:

    • To study the reversions of mutations in dra and drm genes.
    • To analyze simultaneous reversions for thymine requirement (Thy) and thymidine sensitivity (Tdr).
    • To characterize the phenotypic and genotypic changes associated with these reversions.

    Summary:

    • Simultaneous reversions (Thy + Tdr) were observed in Bacillus thuringiensis mutants, with frequencies ranging from 10(-4) to 10(-8).
    • These double revertants consistently displayed rough (R) colony morphology, unlike the smooth (S) parental strains.
    • Selection methods targeting rough variants or specific resistance markers efficiently identified Thy + Tdr revertants.

    Impact:

    • Reveals a correlation between specific genetic reversions and colony morphology in Bacillus thuringiensis.
    • Provides insights into the genetic mechanisms underlying thymine metabolism and thymidine sensitivity.
    • Establishes selection strategies for identifying specific mutant phenotypes in bacterial research.

    Related Experiment Videos