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Related Experiment Videos

The Staphylococcus aureus receptor for fibronectin.

D J Bibel, R Aly, H R Shinefield

    The Journal of Investigative Dermatology
    |June 1, 1983
    PubMed
    Summary

    Staphylococcus aureus adherence to nasal cells is reduced by fibronectin. Ribitol teichoic acid on S. aureus acts as the fibronectin receptor, mediating bacterial binding to keratinized cells.

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    Area of Science:

    • Microbiology
    • Cell Biology
    • Biochemistry

    Background:

    • Staphylococcus aureus is a common pathogen that can colonize nasal epithelial cells.
    • Fibronectin is a protein involved in cell adhesion and tissue repair.
    • Understanding bacterial adherence mechanisms is crucial for developing strategies to prevent infections.

    Purpose of the Study:

    • To identify the specific binding site of Staphylococcus aureus for human fibronectin.
    • To elucidate the role of bacterial cell wall components in fibronectin-mediated adherence.

    Main Methods:

    • Biologic assays were used to measure the adherence of S. aureus to human nasal epithelial cells.
    • Fibronectin treatment was applied to S. aureus to assess its effect on adherence.
    • Inhibition assays were performed using various S. aureus cell wall components.

    Main Results:

    • Fibronectin treatment significantly reduced S. aureus adherence to high granular and fully keratinized nasal cells.
    • N-acetyl-D-glucosamine, N-acetyl muramic acid, and protein A did not inhibit fibronectin binding.
    • Ribitol teichoic acid inhibited fibronectin binding, and fibronectin neutralized this effect.

    Conclusions:

    • Ribitol teichoic acid on Staphylococcus aureus serves as the receptor for human fibronectin.
    • This interaction is important for bacterial adherence to keratinized epithelial cells.

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