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The nerve growth factor receptor on PC12 cells: interconversion between two forms with different binding properties.

T Block, M Bothwell

    Journal of Neurochemistry
    |June 1, 1983
    PubMed
    Summary
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    PC12 cells have two types of nerve growth factor (NGF) receptors. Membrane fusion converted fast NGF receptors to slow ones, suggesting cytoskeletal involvement in receptor behavior.

    Area of Science:

    • Cell Biology
    • Neuroscience
    • Biochemistry

    Background:

    • PC12 cells express two classes of nerve growth factor (NGF) receptors: fast and slow, differing in binding kinetics and cellular association.
    • Fast receptors are rapidly released, while slow receptors exhibit slower kinetics and cytoskeletal association.
    • PC12 cell plasma membranes primarily contain fast NGF receptors.

    Purpose of the Study:

    • To investigate the behavior of NGF receptors after transfer between different cell types.
    • To determine if NGF receptor kinetics can be altered by cell fusion and cytoskeletal interactions.
    • To explore the role of cytoskeleton in modulating NGF receptor function.

    Main Methods:

    • PC12 cell plasma membranes were isolated.
    • Membranes were fused with receptorless 3T3 cells using polyethylene glycol.

    Related Experiment Videos

  • Immunofluorescence microscopy with a monoclonal antibody (C10-2) tracked PC12 membrane and NGF receptor incorporation.
  • NGF receptor binding and release kinetics were analyzed in fused cells.

    • Main Results:

    • Fused 3T3 cells acquired PC12 cell membranes, with up to 90% of 3T3 cells successfully integrating the membrane.
    • The incorporated NGF receptors in 3T3 cells predominantly exhibited slow receptor behavior.
    • PC12 membrane integration resulted in a conversion from fast to slow NGF receptor kinetics.

    Conclusions:

    • NGF receptor kinetics are influenced by cellular context, particularly cytoskeletal association.
    • The conversion of fast to slow NGF receptors upon membrane fusion suggests a role for the cytoskeleton in modulating receptor dynamics.
    • Differences in NGF receptor populations between PC12 and 3T3 cells may reflect distinct cytoskeletal organizations.