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The cytoskeleton in tumor cells.

P Bannasch, H Zerban, D Mayer

    Pathology, Research and Practice
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Cytoskeletal intermediate filaments in tumor cells maintain their features during neoplastic transformation, aiding in tumor diagnosis and classification. Their specific patterns help distinguish tumor origins and types, including metastases.

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    Area of Science:

    • Oncology
    • Cell Biology
    • Biochemistry

    Background:

    • Cytoskeletal components, particularly intermediate filaments, are crucial for cell structure and differentiation.
    • Antibodies to intermediate filament proteins are established markers for distinguishing various cell and tissue types.
    • Neoplastic transformation can alter cellular structures, including the cytoskeleton, impacting tumor behavior and diagnosis.

    Purpose of the Study:

    • To investigate the behavior and diagnostic relevance of cytoskeletal intermediate filaments in neoplastic cells.
    • To explore the role of intermediate filaments in tumor classification and determining histogenetic origin.
    • To understand how cytoskeletal alterations contribute to neoplastic cell transformation.

    Main Methods:

    • Electron microscopy to visualize cellular ultrastructure.

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  • Immunocytochemical methods and immunofluorescence microscopy using antibodies against intermediate filament proteins.
  • Analysis of tumor cells growing both in vitro and in vivo.

    • Main Results:

    • Cytoskeletal organization in vitro tumor cells is highly variable.
    • Regularities in intermediate filament organization are observed in tumor cells growing in vivo, aiding diagnosis.
    • Ultrastructural and immunological features of intermediate filaments are generally maintained during neoplastic transformation in vivo.
    • An accumulation of various intermediate filaments (prekeratin, vimentin, glial fibrillar protein) is observed in different tumor types.
    • The role of contractile proteins like actin in metastasis is not consistently supported by recent findings.

    Conclusions:

    • Immunofluorescence microscopy using cytoskeletal protein antibodies is a powerful tool for tumor classification and differential diagnosis.
    • Intermediate filament patterns in vivo tumors provide valuable information for determining histogenetic origin, especially distinguishing epithelial from mesenchymal tumors and metastases.
    • Further research into intermediate filament alterations in tumors may elucidate molecular mechanisms of neoplastic transformation.