Human papovavirus infection of an SV40-transformed human embryonic kidney cell line

Human embryonic kidney cells were transformed at their third passage with an origin-defective mutant of SV40, 1-11. One clone of these cells, SV1, demonstrated several phenotypes of transformation including high-density growth and anchorage independence. The SV1 cells also were 100% T antigen-positive when tested by immunofluorescence and immunoprecipitation assays. These cells with the SV40 T protein were able to support the persistent replication of BK viral DNA but did not support virus multiplication. The SV1 cells would also support the replication of the Sau 3A A fragment of the BK genome cloned into pBR 322. However, SV1 cells would not support multiplication of JC virus or replication of JC DNA.