Related Experiment Video

Updated: Oct 23, 2026

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes
09:35

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

Published on: February 8, 2009

Electron microscopic evidence for parainfluenza type III/retrovirus phenotypically mixed particles

M Rudolph

    Archiv Fur Geschwulstforschung
    |January 1, 1983
    PubMed

    Insights

    This study found evidence of both a type-D retrovirus (PMFV) and parainfluenza virus type III within the same human fibroblast cells. Researchers also observed rare instances of mixed viral particles, suggesting co-infection.

    Area of Science:

    • Virology
    • Cell Biology
    • Electron Microscopy

    Background:

    • Previous studies identified type-D retrovirus (PMFV) and parainfluenza virus type III infections in human fibroblast cell lines.
    • Malignant permanent human fibroblast sublines were utilized for investigation.

    Purpose of the Study:

    • To investigate the co-existence of PMFV and parainfluenza virus type III within infected human fibroblast cells.
    • To identify viral structural elements of both viruses in the same cell using electron microscopy.

    Main Methods:

    • Utilized electron microscopy for high-resolution imaging of infected human fibroblast sublines.
    • Performed detailed ultrastructural analysis to identify viral components.

    Main Results:

    • Confirmed the presence of "viral structural elements" of both PMFV and parainfluenza virus type III within the same cells.
    • Observed rare instances of mixed parainfluenza type III/PMFV particles via electron microscopy.

    Conclusions:

    • Provides electron microscopic evidence for the co-infection of human fibroblast cells by PMFV and parainfluenza virus type III.
    • Suggests the potential for the formation of mixed viral particles during co-infection.

    More Related Videos

    Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting08:40

    Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

    Published on: March 1, 2019

    Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses08:10

    Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

    Published on: January 15, 2020

    Related Concept Videos

    Viral Recombination00:57

    Viral Recombination

    Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
    Retroviruses02:33

    Retroviruses

    Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
    Influenza01:27

    Influenza

    Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...