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Changes in stem cell compartments in mice after hydroxyurea.
Cell and Tissue Kinetics
|March 1, 1978
Summary
Hydroxyurea injection minimally impacts hematopoietic stem cells (CFUs), causing only a temporary dip. Levels rebound significantly, particularly in spleen and blood, increasing radioresistance in mice.
Area of Science:
- Hematology
- Cell Biology
- Pharmacology
Background:
- Hydroxyurea is a chemotherapy agent used to treat myeloproliferative disorders.
- Its effect on hematopoietic stem cells (CFUs) and subsequent radioresistance is not fully understood.
Purpose of the Study:
- To investigate the impact of hydroxyurea on CFUs in mice.
- To assess the resulting changes in radioresistance.
Main Methods:
- Mice received a single injection of hydroxyurea.
- CFU levels in femur, spleen, and blood were monitored over time.
- Mouse radioresistance was assessed post-injection.
Main Results:
- Hydroxyurea killed only ~10% of S-phase CFUs.
- Femur CFUs decreased ~50% initially, then overshot normal levels after 8 days.
- Spleen CFUs showed a 300% overshoot by day 3.
- Blood CFUs rapidly disappeared and reappeared, followed by an overshoot.
- Mouse radioresistance increased up to 4 days post-injection.
Conclusions:
- Hydroxyurea has a limited and transient effect on CFU populations.
- Significant CFU overshoot occurs in spleen and blood after hydroxyurea administration.
- Increased radioresistance in mice correlates with hydroxyurea treatment, peaking around day 4.