Covalent binding of cefotaxime to human serum albumin

The covalent binding ratio of cephalosporins (CEZ, CER, CET and CTX) to human serum albumin was examined at pH 7 and pH 10. The antibiotic equivalents were larger at pH 10 than at pH 7. The degree in the binding ratio of the 4 cephalosporins was CET greater than CER greater than CTX greater than CEZ at pH 7 and CER greater than CET greater than CTX greater than CEZ at pH 10.