Urinary adenylate kinase isoenzyme pattern in patients with myocardial infarction

Adenylate kinase was purified in pooled urinary samples from patients with uncomplicated myocardial infarction. The purification procedure included ammonium sulfate precipitation and column chromatographic steps. It was necessary to stabilize the enzyme during purification with 2-mercaptoethanol and AMP. Polyacrylamidgelelectrophoresis in sodium dodecanyl sulfate revealed the release of 4 different isoenzymes of AK into urine from patients with myocardial infarction. The molecular weights of these isoenzymes were estimated to be 21,000; 24,000; 33,000 and 36,000, respectively.