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Schistosoma mansoni: surface membrane isolation by polycationic beads.

I M Cesari, G Torpier, A Capron

    Experimental Parasitology
    |August 1, 1983
    PubMed
    Summary

    Researchers developed a rapid method to isolate Schistosoma mansoni surface membranes using polycationic beads. This technique yields highly pure membrane fractions, offering a valuable tool for studying parasite surface proteins and functions.

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    Area of Science:

    • Parasitology
    • Biochemistry
    • Molecular Biology

    Background:

    • Schistosoma mansoni is a parasitic flatworm causing schistosomiasis.
    • Understanding the parasite's surface membrane is crucial for developing treatments.
    • Current isolation methods can be time-consuming and yield impure fractions.

    Purpose of the Study:

    • To develop a rapid and efficient method for isolating the Schistosoma mansoni surface membrane complex.
    • To assess the purity and characteristics of the isolated membrane fractions.
    • To evaluate the utility of this method for biochemical analysis.

    Main Methods:

    • Isolation of Schistosoma mansoni surface membranes by binding polycationic beads to the worm surface.
    • Use of a sucrose- or sorbitol-acetate buffer at pH 5.0 and 4°C.

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  • Measurement of [3H]cholesterol/[14C]arachidonic acid incorporation ratios.
  • Assay of specific enzyme activities: alkaline phosphatase, Type I phosphodiesterase, and Ca2+-adenosine triphosphatase.

    • Main Results:

    • The bead-based isolation method was significantly faster (30 minutes) than traditional techniques.
    • Isolated membrane fractions demonstrated comparable or greater purity than those obtained via sucrose gradient centrifugation.
    • The procedure likely exposed the cytoplasmic surfaces of the membrane fractions.

    Conclusions:

    • The polycationic bead method provides a rapid, efficient, and high-purity isolation of Schistosoma mansoni surface membranes.
    • This technique facilitates biochemical studies of parasite surface components.
    • The exposed cytoplasmic surfaces may enable further functional or structural investigations.