Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Secretin: specific binding to rat brain membranes.

R T Fremeau, R T Jensen, C G Charlton

    The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
    |August 1, 1983
    PubMed
    Summary

    Researchers identified specific, high-affinity secretin binding sites in rat brain membranes. These secretin receptors are distributed unevenly across brain regions and can be studied using radiolabeled secretin.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Barium ion sensing with IPG K<sup>+</sup> molecular probes.

    The Analyst·2025
    Same author

    Fluorescence imaging of individual ions and molecules in pressurized noble gases for barium tagging in <sup>136</sup>Xe.

    Nature communications·2024
    Same author

    Demonstration of event position reconstruction based on diffusion in the NEXT-white detector.

    The European physical journal. C, Particles and fields·2024
    Same author

    Mechanism of internal fertilization in Pegea socia (Tunicata, Thaliacea), a salp with a solid oviduct.

    Journal of morphology·2018
    Same author

    Effects of Coralliophila violacea on tissue loss in the scleractinian corals Porites spp. depend on host response.

    Diseases of aquatic organisms·2016
    Same author

    A retrospective review of treatment and response of high-risk mast cell tumours in dogs.

    Veterinary and comparative oncology·2014

    Area of Science:

    • Neuroscience
    • Pharmacology
    • Biochemistry

    Background:

    • Secretin, a gastrointestinal hormone, has known roles outside the digestive system.
    • The presence and characteristics of secretin receptors in the brain are not fully understood.

    Purpose of the Study:

    • To investigate the binding of [125I]secretin to rat brain membranes.
    • To characterize the properties and distribution of potential secretin binding sites in the rat brain.

    Main Methods:

    • In vitro binding assays using radiolabeled secretin ([125I]secretin).
    • Pharmacological characterization with various peptides and guanine nucleotides.
    • Regional distribution studies across different rat brain areas.

    Main Results:

    Related Experiment Videos

    • High-affinity (KD = 0.2 nM), specific, saturable, and reversible binding of [125I]secretin was observed.
    • Binding sites were most abundant in the cerebellum, followed by cortex, thalamus, striatum, hippocampus, and hypothalamus, with lowest levels in the midbrain and medulla/pons.
    • Only secretin, not other tested peptides, inhibited binding, and guanine nucleotides modulated high-affinity binding.

    Conclusions:

    • Rat brain membranes possess high-affinity binding sites specific for secretin.
    • These findings suggest the existence of functional secretin receptors in the brain.
    • [125I]secretin is a valuable tool for investigating secretin receptor kinetics, stoichiometry, specificity, and distribution.