Mechanosensory projections to cuneate, gracile, and external cuneate nuclei in a tree squirrel (fox squirrel, Sciurus...

The distribution and organization of mechanosensory projections to the cuneate, gracile and external cuneate nuclei were mapped in tree squirrels anesthetized with ketamine and urethane. Tungsten microelectrodes were used to record unit and unit cluster responses to mechanical stimulation. Most responses in the gracile nucleus were to stimulation of skin and hairs of the tail, hind foot and leg, and trunk, in that order going from dorsal to ventral in the nucleus. A similar distribution of responses was found in the cuneate nucleus to stimulation of forelimb, neck and pinna going from dorsomedial to ventrolateral in the nucleus. Some responses to stimulation or movement of subcutaneous tissue were found in the cuneate and gracile nuclei. Responses in the external cuneate were to stimulation of deep-lying tissues in the hand medially, the lower arm centrally and the shoulder and trunk laterally. The pattern of projections in the tree squirrel differed most strikingly from that seen in raccoons and opossums in the relatively small extent of projections from the glabrous skin of the forepaws which were concentrated in a small region near the obex in squirrels. In contrast, there was a large representation of hairy receptive fields located on the forelimb in all regions of the squirrel cuneate nucleus. Otherwise, the somatotopic organization of mechanosensory projections to these dorsal column nuclei in tree squirrels was similar to that reported in other mammals.