Effect of ozone on DNA-repair deficient mutants of Bacillus subtilis

The wild type strains and DNA-repair deficient mutant strains of B. subtilis were exposed to ozone. The results show that the lethal effect of ozone is greatly influenced by factors such as ozone concentration, treatment time, growth phase, and treatment of medium. The recA and recC loci seem to be most important and perhaps the polA and uvrA loci may also be required, but the recB and recD loci are not required for the repair of DNA-damase induced by ozone in B. subtilis.