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Genetic mapping of the mouse proto-oncogene c-sis to chromosome 15
Science (New York, N.Y.)
|August 26, 1983
Abstract:
The mouse homolog (c-sis) of the transforming gene of the simian sarcoma virus was mapped to chromosome 15 by the Southern blot analysis of DNA's from hamster-mouse somatic cell hybrids. Alterations in c-sis expression may thus play a role in the various murine neoplastic diseases characterized by rearrangements or duplications of chromosome 15.
Insights
The mouse c-sis gene, linked to cancer, was located on chromosome 15 using DNA analysis in hybrid cells. This finding suggests c-sis alterations may contribute to mouse cancers involving chromosome 15.
Area of Science:
- Genetics
- Molecular Biology
- Oncology
Background:
- The simian sarcoma virus contains a transforming gene, c-sis.
- Gene mapping is crucial for understanding genetic diseases and cancer.
- Somatic cell hybridization is a technique used for gene mapping.
Purpose of the Study:
- To map the mouse homolog of the simian sarcoma virus transforming gene (c-sis).
- To investigate the potential role of c-sis in murine neoplastic diseases.
Main Methods:
- Southern blot analysis of DNA from hamster-mouse somatic cell hybrids.
- Gene mapping techniques.
Main Results:
- The mouse c-sis gene was successfully mapped to chromosome 15.
- This localization was determined through the analysis of DNA from somatic cell hybrids.
Conclusions:
- Alterations in c-sis gene expression may be implicated in murine neoplastic diseases.
- The study highlights the significance of chromosome 15 rearrangements and duplications in the context of mouse cancer development.