Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Na+- and K+-dependent adenosine triphosphatase changes in multiple sclerosis plaques.

H E Hirsch, M E Parks

    Annals of Neurology
    |June 1, 1983
    PubMed
    Summary

    Activity of the sodium pump (Na+- and K+-dependent adenosine triphosphatase) is significantly reduced in active multiple sclerosis plaques. Enzyme activity recovers in older plaques, suggesting reversible changes in this mechanism during MS.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Simultaneous binding of a polyamide dimer and an oligonucleotide in the minor and major grooves of DNA.

    Bioorganic & medicinal chemistry·1996
    Same author

    Cyclic polyamides for recognition in the minor groove of DNA.

    Proceedings of the National Academy of Sciences of the United States of America·1995
    Same author

    Fluorometric oxidase assays: pitfalls caused by action of ultraviolet light on lipids.

    Analytical biochemistry·1982
    Same author

    The ratio of 3-hydroxyacyl-CoA dehydrogenase to lipoamide dehydrogenase activity in individual muscle fibers: mitochondrial specialization for source of energy.

    Journal of neuroscience research·1982
    Same author

    Sarcolemmal protein profiles from cultured myotubes and myoblasts.

    Experimental neurology·1981
    Same author

    Fibrinolytic activity of plaques and white matter in multiple sclerosis.

    Journal of neuropathology and experimental neurology·1981

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Pathology

    Background:

    • Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system.
    • The sodium-potassium pump (Na+- and K+-dependent adenosine triphosphatase) plays a crucial role in maintaining neuronal function.
    • Understanding enzymatic changes in MS lesions is vital for elucidating disease mechanisms.

    Purpose of the Study:

    • To investigate the activity of Na+- and K+-dependent adenosine triphosphatase in active and inactive multiple sclerosis plaques.
    • To compare enzyme activity in MS lesions with unaffected white matter and control samples.

    Main Methods:

    • Microdissection of specific plaque regions from MS patient samples.
    • Histological assessment of plaque activity (active vs. inactive).

    Related Experiment Videos

  • Biochemical assay of Na+- and K+-dependent adenosine triphosphatase activity.

    • Main Results:

    • Significantly reduced Na+- and K+-dependent adenosine triphosphatase activity was observed in active MS plaques.
    • ATPase activity was higher in old, chronic plaques compared to unaffected white matter.
    • No significant difference in ATPase activity was found between control white matter and normal-appearing white matter from MS patients.

    Conclusions:

    • Reversible alterations in the sodium pump mechanism may be implicated in the pathophysiology of multiple sclerosis.
    • The findings suggest a dynamic change in ion transport during the course of MS lesions.
    • Further research into the sodium pump's role could offer new therapeutic targets for MS.