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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
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High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

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Clinical applications of restriction endonuclease analysis

J A Phillips

    Birth Defects Original Article Series
    |January 1, 1983
    PubMed

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    Restriction Enzymes01:11

    Restriction Enzymes

    Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
    The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...