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Updated: Sep 21, 2026
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
Alteration of phenotype of 'regressor' avian sarcoma cells following treatment with phorbol ester
Abstract:
Tumor cells which are cultured from the regressor phase of avian sarcoma virus (ASV)-induced tumor growth are deficient with respect to ability to produce progeny transforming virus. In addition, such cells are relatively unreactive with anti-viral antibody and are unable to elaborate, into the medium, antigens which are stimulatory to the sensitized lymphocytes of virus-injected hosts. These deficits can be overcome by treatment of the cells with the tumor promoter, phorbol myristate acetate (PMA).
Insights
Tumor cells from avian sarcoma virus (ASV)-induced tumors show reduced virus production and immune response. Treatment with phorbol myristate acetate (PMA) can restore these functions, offering insights into tumor virology.
Area of Science:
- Virology
- Immunology
- Oncology
Background:
- Tumor cells from the regressor phase of avian sarcoma virus (ASV)-induced tumors exhibit functional deficits.
- These deficits include impaired progeny virus production and reduced immune cell stimulation.
Purpose of the Study:
- To investigate the functional deficiencies of ASV-induced tumor cells during the regressor phase.
- To determine if phorbol myristate acetate (PMA) can restore these deficient functions.
Main Methods:
- Culturing tumor cells from the regressor phase of ASV-induced tumors.
- Assessing progeny virus production.
- Evaluating reactivity with anti-viral antibody.
- Measuring antigen elaboration stimulating sensitized lymphocytes.
- Treating cells with phorbol myristate acetate (PMA).
Main Results:
- Tumor cells from the regressor phase showed reduced ability to produce transforming virus.
- These cells were less reactive to anti-viral antibodies and did not elaborate stimulatory antigens.
- PMA treatment successfully overcame these functional deficits.
Conclusions:
- ASV-induced tumor cells in the regressor phase have specific functional impairments.
- PMA can reverse these impairments, suggesting a role for tumor promoters in viral oncogenesis.
- Findings provide insights into tumor cell biology and potential therapeutic targets.
