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Detergent solubilisation of rat liver particulate cyclic AMP phosphodiesterase
The International Journal of Biochemistry
|January 1, 1983
Abstract:
Detergent solubilisation of particulate rat liver low Km cyclic AMP phosphodiesterase in the presence of protease inhibitors yields a form of the enzyme with a larger molecular weight than the form solubilised by protease treatment. The detergent solubilised enzyme could be partially purified by anion exchange chromatography. It displayed a marked tendency to precipitate from solution when detergent was removed.
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