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The oxidative burst and related phenomena in mouse macrophages elicited by different sterile inflammatory stimuli
Abstract:
Mouse peritoneal macrophages (MPM) elicited by paraffin oil (PO) or thioglycollate (TG) were compared in their capacity to generate oxidative burst (OB) products such as superoxide ion (O2-) and hydrogen peroxide (H2O2) and lyse target erythrocytes. Strong OB stimulants such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA), opsonized zymosan; and wheat-germ agglutinin (WGA) were found to stimulate O2 and H2O2 production by both PO- and TG-elicited MPM. PO-elicited MPM exhibited a vigorous OB response compared to TG-elicited MPM and showed a higher degree of self iodination in the presence of 125I. Macrophage-mediated cytolysis of 51Cr-labeled red blood cells, following OB stimulation, was revealed by PO-elicited MPM triggered by TPA, opsonized zymosan, WGA, and Concanavalin A. TG-elicited MPM failed to respond to TPA and opsonized zymosan. In the presence of horseradish peroxidase, both PO- and TG-elicited MPM exhibited augmented cytocidal activities upon stimulation with TPA, whereas catalase abrogated the capacity of TPA-stimulated MPM to lyse red blood cells, which may suggest the involvement of H2O2 in the lytic process.
Insights
Paraffin oil-elicited macrophages show a stronger oxidative burst and better erythrocyte lysis than thioglycollate-elicited ones. Hydrogen peroxide (H2O2) appears crucial for this macrophage-mediated cytolysis.
Area of Science:
- Immunology
- Cell Biology
Background:
- Mouse peritoneal macrophages (MPM) are crucial immune cells.
- Different eliciting agents can alter macrophage function.
Purpose of the Study:
- To compare oxidative burst (OB) and cytolytic activity of MPM elicited by paraffin oil (PO) versus thioglycollate (TG).
- To investigate the role of OB products, like superoxide ion (O2-) and hydrogen peroxide (H2O2), in macrophage-mediated erythrocyte lysis.
Main Methods:
- Eliciting MPM using PO or TG.
- Stimulating macrophages with OB inducers like TPA, zymosan, and WGA.
- Measuring O2- and H2O2 production.
- Assessing macrophage-mediated lysis of 51Cr-labeled erythrocytes.
- Investigating the effect of horseradish peroxidase and catalase.
Main Results:
- PO-elicited MPM demonstrated a more vigorous OB response and higher self-iodination compared to TG-elicited MPM.
- PO-elicited MPM effectively lysed erythrocytes when stimulated with TPA, zymosan, WGA, and Concanavalin A.
- TG-elicited MPM showed limited response to TPA and zymosan stimulation.
- Hydrogen peroxide (H2O2) involvement in cytolysis was suggested by the effects of horseradish peroxidase and catalase.
Conclusions:
- PO is a more potent elicitor of macrophages with enhanced oxidative burst and cytolytic functions compared to TG.
- Hydrogen peroxide (H2O2) plays a significant role in the cytolytic process mediated by stimulated macrophages.