Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Colonial opacity variation in Mycoplasma pulmonis.

A Liss, R A Heiland

    Infection and Immunity
    |September 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Influence of Klebsiella pneumoniae and quinolone treatment on prognosis in patients with pancreatic cancer.

    The British journal of surgery·2021
    Same author

    Professional competencies and work-related support in relation to periodontal therapy and work satisfaction: A questionnaire study among Swedish Dental Hygienists.

    International journal of dental hygiene·2017
    Same author

    The chicken RelB transcription factor has transactivation sequences and a tissue-specific expression pattern that are distinct from mammalian RelB.

    Molecular cell biology research communications : MCBRC·2001
    Same author

    One manufacturer's approach to using nucleic acid testing for enhanced plasma-product safety.

    Transfusion medicine reviews·2001
    Same author

    Primary sensory neuron survival following targeted administration of nerve growth factor to an injured nerve.

    Scandinavian journal of plastic and reconstructive surgery and hand surgery·1999
    Same author

    Enzymological features of aromatic amino acid biosynthesis reflect the phylogeny of mycoplasmas.

    Journal of general microbiology·1987

    Mycoplasma pulmonis exhibits colonial opacity variation, with transparent variants arising from opaque cultures. Serum type influences opacity visualization and growth, while DNA and protein profiles distinguish variants.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Pathogen Research

    Background:

    • Mycoplasma pulmonis is a significant murine pathogen.
    • Colonial morphology, including opacity, can vary within bacterial populations.

    Purpose of the Study:

    • To investigate and characterize colonial opacity variants in Mycoplasma pulmonis.
    • To determine the stability and interconversion rates of these variants.
    • To explore factors influencing opacity expression and underlying molecular differences.

    Main Methods:

    • Isolation and selection of stable opaque and transparent colonial variants.
    • Cultivation in media supplemented with different serum types (horse, sheep, fetal bovine).
    • Analysis of protein profiles using SDS-PAGE.

    Related Experiment Videos

  • Analysis of DNA fragment patterns using agarose gel electrophoresis after endonuclease digestion.

    • Main Results:

    • Stable opaque and transparent variants of Mycoplasma pulmonis were identified.
    • Transparent variants arose from opaque cultures at a rate of 1.2 x 10(-8) per CFU per generation.
    • Serum source significantly impacted opacity visualization and plating efficiency.
    • Distinct polypeptide profiles and DNA fragment patterns differentiated variants within specific strains.
    • These molecular differences were stable across different serum supplements.

    Conclusions:

    • Colonial opacity in Mycoplasma pulmonis is a heritable trait with distinct molecular underpinnings.
    • Environmental factors, such as serum composition, modulate phenotype expression.
    • Opaque and transparent variants possess stable genetic and proteomic differences.