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The rabbit uteroglobin gene. Structure and interaction with the progesterone receptor
The Journal of Biological Chemistry
|September 10, 1983
Summary
This study analyzes the rabbit uteroglobin gene regulation, revealing its promoter region has multiple high-affinity progesterone receptor binding sites. This provides insights into progesterone
Area of Science:
- Molecular Biology
- Genetics
- Endocrinology
Background:
- Uteroglobin gene regulation in rabbit endometrium serves as a model for progesterone action in mammals.
- The uteroglobin gene has been cloned, sequenced, and its exons mapped to protein functional domains.
Purpose of the Study:
- To elucidate the mechanism of progesterone action on uteroglobin gene regulation.
- To identify regulatory elements within the uteroglobin gene promoter region.
Main Methods:
- Gene sequencing and comparison with protein structure.
- S1 mapping to define transcription initiation sites.
- Nitrocellulose filter binding assays to identify receptor-DNA interactions.
Main Results:
- Exon-domain correspondence and splice junction analysis were performed.
- Identified the primary RNA polymerase initiation site and several minor sites.
- Discovered multiple high-affinity progesterone receptor binding sites in the 5' flanking and promoter regions of the uteroglobin gene.
Conclusions:
- The 5' region of the uteroglobin gene contains key elements for progesterone receptor binding.
- Understanding these interactions is crucial for deciphering steroid hormone regulation mechanisms.