Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lymphocyte adenylate cyclase and human aging.

J F Krall, M Connelly-Fittingoff, M L Tuck

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
    |September 1, 1983
    PubMed
    Summary

    Adenylate cyclase activity in human lymphocytes decreases with age. This decline, linked to the cyclic AMP (cAMP) system, may explain age-related immune response changes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Familial obesity, sympathetic activation and blood pressure level.

    Blood pressure·2002
    Same author

    Differences in mechanisms between weight loss-sensitive and -resistant blood pressure reduction in obese subjects.

    Hypertension research : official journal of the Japanese Society of Hypertension·2001
    Same author

    Long-term fructose feeding impairs vascular relaxation in rat mesenteric arteries.

    American journal of hypertension·2001
    Same author

    Selective aspects of the insulin resistance syndrome.

    Current opinion in nephrology and hypertension·2001
    Same author

    Weight reduction and pharmacologic treatment in obese hypertensives.

    American journal of hypertension·2001
    Same author

    Inhibitors of arachidonic acid metabolism have variable effects on calcium signaling pathways.

    American journal of hypertension·2001

    Area of Science:

    • Immunology
    • Cellular signaling
    • Biochemistry

    Background:

    • Adenylate cyclase is a key enzyme in cellular signaling pathways.
    • Beta-adrenergic stimulation and guanine nucleotides (GTP) modulate adenylate cyclase activity.
    • Cyclic AMP (cAMP) production is crucial for various cellular functions, including immune responses.

    Purpose of the Study:

    • To investigate the age-related changes in human lymphocyte adenylate cyclase activity.
    • To determine the kinetic properties of lymphocyte adenylate cyclase in response to stimulation.
    • To explore the role of GTP-binding proteins in age-associated alterations of the cAMP pathway.

    Main Methods:

    • Enzymatic assay of adenylate cyclase activity using [32P]ATP conversion to [32P]cAMP in isolated human lymphocytes.
    • Stimulation of the enzyme with isoproterenol (a beta-adrenergic agonist) and Gpp[NH]p (a GTP analog).
    • Kinetic analysis (Michaelis-Menten) of enzyme activity and determination of activation constants (Kact) in different age groups.

    Main Results:

    • Adenylate cyclase activity, stimulated by isoproterenol and/or Gpp[NH]p, significantly decreased with increasing age in human subjects.
    • Isoproterenol and Gpp[NH]p exhibited synergistic effects on enzyme activity, following Michaelis-Menten kinetics.
    • The sensitivity (Kact) of the enzyme to isoproterenol remained consistent across age groups, indicating preserved receptor function.

    Conclusions:

    • A deficiency in the lymphocyte adenylate cyclase system, distal to the beta-adrenergic receptor, contributes to age-related immune decline.
    • The findings highlight a potential molecular basis for the deterioration of cAMP-mediated immune responses in the elderly.
    • GTP-binding coupling factors play an essential role in the intact lymphocyte adenylate cyclase system.

    Related Experiment Videos