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Blue light effect on proton pumping by bacteriorhodopsin.

K Ohno, R Govindjee, T G Ebrey

    Biophysical Journal
    |August 1, 1983
    PubMed
    Summary
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    Blue light inhibits proton pumping in bacteriorhodopsin by interacting with a specific intermediate (M412). This interaction, involving a slowly decaying component, may reverse proton movement direction.

    Area of Science:

    • Biophysics
    • Photochemistry
    • Membrane protein function

    Background:

    • Bacteriorhodopsin is a light-driven proton pump.
    • Proton pumping is initiated by light absorption and involves photocycle intermediates.
    • The M412 intermediate plays a key role in the bacteriorhodopsin photocycle.

    Purpose of the Study:

    • To investigate the effect of blue light on proton pumping initiated by orange light in bacteriorhodopsin vesicles.
    • To identify the specific photocycle intermediate responsible for blue light's inhibitory effect.
    • To correlate proton movement with a specific photochemical intermediate.

    Main Methods:

    • Flash photolysis experiments using orange and blue light.
    • Kinetic analysis of proton pumping.

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  • Spectroscopic characterization of photocycle intermediates.

    • Main Results:

    • A subsequent blue flash diminishes proton pumping initiated by an orange flash.
    • The M412 intermediate, formed by orange light, mediates the blue light effect.
    • Kinetic analysis reveals that only the slowly decaying component of M412 is involved in reducing proton movement.
    • This is the first reported correlation between proton movement and a specific bacteriorhodopsin intermediate.

    Conclusions:

    • Blue light inhibits bacteriorhodopsin proton pumping by interacting with the slowly decaying M412 intermediate.
    • This interaction may cause a reversal of proton movement direction.
    • Provides novel insights into the mechanism of proton translocation in bacteriorhodopsin.