Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Synaptic interactions in the GABA system during postnatal development in retina.

P C Madtes, D A Redburn

    Brain Research Bulletin
    |June 1, 1983
    PubMed
    Summary

    The GABA uptake system in newborn rabbit retinas influences GABA receptor development. Blocking this system with nipecotic acid increases receptor binding, suggesting GABA acts as a trophic factor.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of chaotropic anions on bicuculline inhibition of high affinity ?-aminobutyric acid binding in bovine retina and rat brain membranes.

    Neurochemistry international·2010
    Same author

    N-methyl-D-aspartate-mediated glutamate toxicity in the developing rabbit retina.

    Journal of neuroscience research·1997
    Same author

    The temporal and spatial development of corticotropin-releasing factor (CRF) binding sites and CRF afferents in the opossum cerebellum.

    Journal of chemical neuroanatomy·1996
    Same author

    GABA and GABA-A receptors are maximally expressed in association with cone synaptogenesis in neonatal rabbit retina.

    Brain research. Developmental brain research·1996
    Same author

    Developmental neurotransmitters. Signals for shaping neuronal circuitry.

    Investigative ophthalmology & visual science·1996
    Same author

    Expression and downregulation of the GABAergic phenotype in explants of cultured rabbit retina.

    Investigative ophthalmology & visual science·1996

    Area of Science:

    • Neuroscience
    • Developmental Biology
    • Biochemistry

    Background:

    • The retina possesses a GABA uptake system crucial for neurotransmitter regulation.
    • Understanding GABAergic system development is vital for comprehending retinal function.
    • GABA's role as a potential trophic factor in neural development warrants investigation.

    Purpose of the Study:

    • To characterize the GABA uptake system in developing rabbit retinas.
    • To investigate the impact of inhibiting GABA uptake on GABA receptor development.
    • To explore GABA's potential role as a trophic factor during retinal development.

    Main Methods:

    • Biochemical analysis of GABA uptake kinetics in rabbit retinas.
    • Assessment of GABA uptake system activity during postnatal development.
    • Pharmacological inhibition of GABA uptake using nipecotic acid.
    • Quantification of postsynaptic GABA receptors via 3H-muscimol binding assays.

    Main Results:

    • A high-affinity, sodium- and temperature-dependent GABA uptake system is present in newborn rabbit retinas.
    • GABA uptake activity reaches adult levels between postnatal days 6-8.
    • Nipecotic acid treatment significantly inhibited GABA uptake and increased GABA receptor binding before eye opening.

    Conclusions:

    • GABA uptake system activity influences the development of postsynaptic GABA receptors.
    • GABAergic neurotransmission may play a trophic role in the developing rabbit retina.
    • Inhibition of GABA uptake can modulate GABA receptor site maturation.

    Related Experiment Videos