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Molecular cloning of cDNA from foot-and-mouth disease virus C1-Santa Pau (C-S8). Sequence of protein-VP1-coding
Abstract:
cDNA segments copied from the RNA of foot-and-mouth disease virus (FMDV) C1-Santa Pau (isolate C-S8) have been cloned in plasmid pBR322. A 998-bp DNA fragment, that includes the region coding for capsid protein VP1, the carboxy terminus of VP3, and the amino terminus of precursor protein p52 has been sequenced. Comparison of the nucleotide sequence with those from FMDV O1K, A(10)61, A12 and C3 Indaial (Kurz et al., Nucl. Acids Res. 9 (1981) 1919-1931; Kleid et al., Science 214 (1981) 1125-1129; Boothroyd et al., Gene 17 (1982) 153-161; Makoff et al., Nucl. Acids Res. 10 (1982) 8285-8295) indicates extensive variability between the corresponding gene segments, including short insertions and deletions. Base transversions are more frequent than transitions within the VP1 coding segment, but not in the sequence coding for the amino-terminal end of p52. The nucleotide sequence divergence is reflected in variability in both the primary and the predicted higher-order structures of the encoded VP1s.
Insights
Foot-and-mouth disease virus (FMDV) VP1 protein sequences show significant genetic variability. This divergence impacts both the structure and function of FMDV capsid proteins, crucial for viral evolution.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Foot-and-mouth disease virus (FMDV) is a significant pathogen affecting livestock.
- Understanding genetic variability in FMDV is crucial for developing effective control strategies.
- The capsid protein VP1 is a key determinant of FMDV antigenicity and host interaction.
Purpose of the Study:
- To clone and sequence a specific cDNA region of FMDV C1-Santa Pau.
- To analyze the nucleotide sequence variability of FMDV gene segments.
- To compare the genetic diversity of FMDV isolates and its impact on protein structure.
Main Methods:
- Cloning of FMDV cDNA segments into the plasmid pBR322.
- DNA sequencing of a 998-bp fragment encoding VP1, VP3 carboxy terminus, and p52 amino terminus.
- Comparative nucleotide sequence analysis with other FMDV strains (O1K, A(10)61, A12, C3 Indaial).
Main Results:
- A 998-bp DNA fragment from FMDV C1-Santa Pau was successfully sequenced.
- Extensive nucleotide sequence variability, including insertions and deletions, was observed compared to other FMDV strains.
- Base transversions were more frequent than transitions in the VP1 coding region, leading to structural variations in VP1.
Conclusions:
- Significant genetic divergence exists within FMDV gene segments, particularly in the VP1 coding region.
- This sequence variability directly influences the primary and predicted higher-order structures of the VP1 protein.
- The findings highlight the dynamic nature of FMDV evolution and provide insights into viral adaptation.