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Ecotropic MuLV expression in radiation-induced lymphomas of the RF, BALB/c and (BALB/c X RF)F1 mouse strains
Abstract:
Endogenous ecotropic viruses isolated from radiation-induced lymphomatous tissue of BALB/c mice have been shown to consist of a collection of variants as assayed by the mobility of virion structural proteins p30, p15, p12 and gp70 on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Ellis et al., 1980 a). In this study we show that a similar phenomenon occurs in RF mice, but only with regard to p15 and gp70, and not p30 and p12. Using the distinct and unvarying mobility of the RF viral p12 protein on SDS-PAGE as a marker, we show that the RF-derived ecotropic murine leukemia virus (MuLV) is expressed to the exclusion of the BALB/c-derived ecotropic MuLV in F1 hybrid mice of the BALB/c X RF cross, and that variant viruses expressed in F1 radiation-induced lymphomatous tissue display the pattern of variation characteristic of the RF, and not of the BALB/c, strain.
Insights
Endogenous ecotropic murine leukemia viruses (MuLV) from RF mice show variation in proteins p15 and gp70, unlike BALB/c MuLV. Hybrid mice express RF-type MuLV variants, indicating strain-specific viral patterns.
Area of Science:
- Virology
- Immunology
- Genetics
Background:
- Endogenous ecotropic viruses in radiation-induced lymphomatous tissue of BALB/c mice exhibit heterogeneity in structural proteins (p30, p15, p12, gp70) as determined by SDS-PAGE.
- This viral variation suggests complex interactions within the host immune system and viral replication.
Purpose of the Study:
- To investigate the variation patterns of endogenous ecotropic murine leukemia viruses (MuLV) in RF mice compared to BALB/c mice.
- To determine the viral expression and variation characteristics in F1 hybrid mice derived from BALB/c and RF strains.
Main Methods:
- Analysis of virion structural proteins (p30, p15, p12, gp70) from radiation-induced lymphomatous tissue using SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
- Utilizing the distinct mobility of the RF viral p12 protein as a molecular marker.
- Comparing MuLV expression and variation patterns in F1 hybrid mice (BALB/c X RF) with those of the parent strains.
Main Results:
- RF mice exhibit MuLV variation in p15 and gp70 proteins, but not in p30 and p12, contrasting with BALB/c mice.
- F1 hybrid mice exclusively express RF-derived ecotropic MuLV, excluding BALB/c-derived MuLV.
- Variant viruses in F1 hybrid lymphomatous tissue display variation patterns characteristic of the RF strain, not the BALB/c strain.
Conclusions:
- Endogenous ecotropic MuLV populations display strain-specific variation patterns in structural proteins.
- The RF viral p12 protein serves as a reliable marker for identifying MuLV strain origins in hybrid mice.
- Host genetics significantly influence the expression and variation of endogenous MuLV in radiation-induced lymphomas.