Karyotype evolution in a transformed rat cerebral endothelial cell line

Primary cultures of rat microvascular endothelial cells were transformed, in vitro, by exposure to Rous sarcoma virus. Transformed cells were followed and evaluated cytogenetically through numerous passages. Highly specific karyotypic changes in karyotype (both structural and numerical) were documented. These changes became established and intimately involved in further "karyotypic evolution". The findings were reproducible, and when considered in the light of the literature suggest regular patterns of karyotypic change in rat tumors. The in vitro methodology utilized promises to be of practical value in the study of the early stages of malignancy.