Related Experiment Video

Updated: Aug 14, 2026

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
10:24

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Published on: October 28, 2014

pH regulation of divalent/monovalent Ca/K cation transport selectivity by a macrocyclic carrier molecule

A Hriciga, J M Lehn

    Proceedings of the National Academy of Sciences of the United States of America
    |October 1, 1983
    PubMed

    More Related Videos

    A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters07:47

    A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

    Published on: April 20, 2015

    Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+12:30

    Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

    Published on: May 19, 2017

    Related Concept Videos

    The Significance of Membrane Transport01:44

    The Significance of Membrane Transport

    The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
    Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
    Secondary Active Transport01:32

    Secondary Active Transport

    One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
    Feedback Regulation of Calcium Concentration01:27

    Feedback Regulation of Calcium Concentration

    Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
    Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
    Carrier-Mediated Transport01:06

    Carrier-Mediated Transport

    Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
    Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
    Facilitated Diffusion01:16

    Facilitated Diffusion

    The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
    In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
    Active Transport01:14

    Active Transport

    Active transport is a critical biological process that allows cells to move solutes against an electrochemical gradient. This process requires direct energy input and is characterized by its selectivity, saturability, and susceptibility to competitive inhibition.
    Primary active transporters, like Na+, K+ and -ATPase, directly utilize ATP to move ions across the membrane. These transporters play significant roles in various physiological processes. For instance, Na+, K+ and -ATPase maintain...