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Related Experiment Videos

Restriction map of Tn7.

F Gosti-Testu, V Norris, J Brevet

    Plasmid
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    This study maps the Tn7 transposon, which confers resistance to trimethoprim and streptomycin. The detailed restriction enzyme cleavage map aids in understanding Tn7

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Tn7 is a 14 kb transposon.
    • Tn7 confers resistance to trimethoprim (Tp) and streptomycin (Sm).

    Purpose of the Study:

    • To create a detailed cleavage site map of the Tn7 transposon.
    • To facilitate the study of Tn7's structural and genetic organization.

    Main Methods:

    • Restriction enzyme digestion of ColE1 and ColE1::Tn7 plasmids.
    • Analysis of deletion derivatives (pGB2, ColE1::Tn7 delta 6).
    • Mapping using cloned Tn7 fragments in pBR322 (pOB14, pOB15).

    Main Results:

    • A cleavage site map for twenty-two different restriction enzymes was generated for Tn7.
  • Precise localization of restriction sites was achieved using deletion mutants and cloned fragments.

    • Conclusions:

    • The generated map provides a foundational tool for future research on Tn7.
    • Understanding Tn7's structure is crucial for studying antibiotic resistance mechanisms.