Cross antigenicity among enteroviruses as revealed by immunoblot technique

Antigenic relationships of various human and two animal picornaviruses were investigated by the immunoblotting ("Western blot") technique. The viruses included all coxsackievirus B types (1-6), poliovirus types 1-3, several strains of echovirus 11, EMC virus, and FMDV. Antisera included human sera and sera from rabbits hyperimmunized with either purified picornaviruses, viral structural polypeptides (VP8), boiled or "sample-boiled" virions. Group-specific reactions of various extent were observed among the human picornaviruses, but not with EMC virus. These reactions were obtained with human sera (whole serum, IgG- and IgM-fraction) as well as with "monospecific" (neutralization test) rabbit antisera. Among cross reacting polypeptides VP1 was predominant with the notable exception of coxsackie B4, where VP1 (defined according to cleavage pattern) migrates in our gel system as second largest polypeptide. Antisera prepared vs VP1 had neutralizing activity as demonstrated with five different echovirus 11 strains (titers up to 2000). Antisera vs VP1 (and other VP8) exhibited cross-reactivity in the immunoblots. Antisera to the three poliovirus types (and to certain echovirus 11 strains) showed a surprisingly narrow cross-reacting spectrum which--in the case of poliovirus--could not be broadened by additional hyperimmunization of the rabbits with heated poliovirus 2. The significance of these results for a diagnostic ELISA in patients with picornavirus infections is dealt with.