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Updated: Aug 22, 2026
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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
Published on: July 28, 2007
A novel chromatographic procedure for purification of bacterial plasmids
Analytical Biochemistry
|July 1, 1983
Abstract:
A new, rapid procedure for purifying bacterial plasmids with high recovery is described. The sequence of operations consists essentially of treatment with alkali, ribonuclease, and proteinase K, followed by chisam extraction and gel filtration on Sephacryl S-1000, and finally a precipitation step using isopropanol at room temperature. The method gives rather good yields of plasmid DNA of high purity, and lends itself to scaling up.
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