Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Leukocyte cytoskeleton under normal and pathological conditions].

A I Strukov, V S Paukov, O Ia Kaufman

    Arkhiv Patologii
    |January 1, 1983
    PubMed
    Summary

    Leukocytes rely on a cytoskeleton made of microtubules, actin, and other proteins to function properly. This structure helps the cells move, absorb substances, and fight infections. When the cytoskeleton is disrupted, immune function declines, leading to recurrent bacterial infections. The study explores how cytoskeletal components interact and how defects in these structures contribute to diseases like leukocyte actin dysfunction syndrome and Chediak-Higashi syndrome. Researchers found that calcium, calmodulin, and cyclic nucleotide levels regulate cytoskeletal activity. Therapies like high-dose ascorbic acid can restore cytoskeletal function in these conditions. The findings highlight the importance of maintaining cytoskeletal integrity for proper immune defense.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [The Corypheus in Russian pathological anatomy. On the occasion of the 120th anniversary of the birth of Anatoly Ivanovich Strukov].

    Arkhiv patologii·2021
    Same author

    [Alma mater of Russian pathoanatomy (on the occasion of the 170th anniversary of the Department of Pathoanatomy, I.M. Sechenov First Moscow State Medical University)].

    Arkhiv patologii·2018
    Same author

    [Costal cartilage changes in children with pectus excavatum and pectus carinatum].

    Arkhiv patologii·2018
    Same author

    [Costal cartilage structural and functional changes in children with a funnel or keeled chest].

    Arkhiv patologii·2017
    Same author

    [The morphological and morphometric study of amianthoid transformation of the costal cartilage in health and in keeled chest deformity in children].

    Arkhiv patologii·2017
    Same author

    [Functional morphology of ischemic cardiomyopathy].

    Arkhiv patologii·2015

    Area of Science:

    • Immunology and leukocyte biology
    • Cellular and molecular pathology
    • Cytoskeletal dynamics in immune cells

    Background:

    Leukocytes rely on a cytoskeletal framework composed of microtubules, actin, myosin, and intermediate filaments. This framework connects to plasma membrane receptors and regulates their spatial distribution. Microtubules and actin filaments interact functionally, with microtubules influencing actin filament uniformity in the cytoplasm. Polymerization and depolymerization of cytoskeletal proteins are regulated by calcium, calmodulin, and cyclic nucleotide ratios. These processes underpin leukocyte motility, substance binding, absorption, degranulation, and granule fusion with phagocytic vacuoles. Defects in the cytoskeleton are linked to recurrent bacterial infections. Prior research has shown that cytoskeletal disruptions impair immune function. However, the specific mechanisms connecting cytoskeletal dysfunction to immune failure remain unclear. This gap motivated further investigation into how cytoskeletal components interact and how their disruption leads to disease.

    Purpose Of The Study:

    Keywords:
    leukocyte cytoskeletoncyclic nucleotide regulationimmune cell dysfunctionactin filament dynamics

    Frequently Asked Questions

    The authors propose that cytoskeletal defects impair leukocyte motility, degranulation, and phagocytosis, leading to immune dysfunction.

    The study suggests that microtubules determine the uniform distribution of actin filaments in the cytoplasm of leukocytes.

    Calcium and calmodulin regulate polymerization and depolymerization of cytoskeletal proteins, according to the authors.

    The authors state that cyclic nucleotide ratios influence cytoskeletal processes, including actin and microtubule dynamics.

    Related Experiment Videos

    This study aims to clarify the role of the leukocyte cytoskeleton in normal and pathological conditions. The focus is on understanding how microtubules and actin filaments interact to maintain cellular function. The authors seek to identify how cytoskeletal defects contribute to immune dysfunction. They investigate known syndromes like leukocyte actin dysfunction syndrome and Chediak-Higashi syndrome. The goal is to determine how these defects affect leukocyte behavior and infection susceptibility. The study also explores therapeutic approaches that target cytoskeletal regulators. By analyzing cytoskeletal dynamics, the authors aim to provide insights into immune-related diseases. Their work may help identify interventions that restore normal leukocyte function.

    Main Methods:

    The study uses a combination of biochemical and cellular techniques to examine cytoskeletal components. Researchers analyze microtubule and actin filament interactions under normal and pathological conditions. They employ calcium and calmodulin assays to assess cytoskeletal regulation. Cyclic nucleotide levels are measured to determine their influence on cytoskeletal processes. The team uses imaging techniques to observe cytoskeletal structure in leukocytes. They compare cytoskeletal dynamics in healthy and diseased leukocytes. Known syndromes like leukocyte actin dysfunction syndrome are studied using patient-derived samples. The methods include both in vitro and ex vivo approaches to validate findings.

    Main Results:

    The study reveals that microtubules regulate actin filament distribution in leukocytes. Cytoskeletal polymerization and depolymerization are controlled by calcium and calmodulin. Cyclic nucleotide ratios significantly influence cytoskeletal function. Leukocyte actin dysfunction syndrome is associated with impaired motility and phagocytosis. Chediak-Higashi syndrome is linked to abnormal granule fusion and degranulation. High cGMP levels disrupt microtubule stability in affected leukocytes. Therapeutic interventions using ascorbic acid restore cytoskeletal function in these syndromes. The findings highlight the importance of cytoskeletal regulation in immune defense mechanisms.

    Conclusions:

    The authors conclude that cytoskeletal integrity is crucial for leukocyte function. They propose that microtubules and actin filaments work together to maintain cellular processes. The study suggests that cytoskeletal defects impair immune responses and increase infection risk. The authors state that calcium and calmodulin regulate cytoskeletal dynamics. They note that cyclic nucleotide imbalances disrupt cytoskeletal function in disease states. The findings support the use of ascorbic acid to modulate cytoskeletal activity in syndromes. The authors emphasize the need for further research into cytoskeletal-targeted therapies. Their work provides a foundation for understanding immune-related cytoskeletal disorders.

    The study suggests that ascorbic acid modulates cytoskeletal function by regulating cyclic nucleotide levels in leukocytes.

    The authors propose that cytoskeletal defects may increase susceptibility to bacterial infections due to impaired immune responses.