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Cholecystokinin turnover in brain.

J L Meek, M J Iadarola, O Giorgi

|October 16, 1983

Summary

This summary is machine-generated.

Cholecystokinin octapeptide (CCK-8) in the brain is sulfated and has a slow turnover rate. This neuropeptide

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Area of Science:

Neuroscience
Biochemistry
Molecular Biology

Background:

Cholecystokinin octapeptide (CCK-8) is a neuropeptide found in the cerebral cortex.
CCK-8 contains a sulfate ester, a unique feature among mammalian neuropeptides.

Purpose of the Study:

To determine the turnover rate of CCK-8 in the rat brain.
To investigate the formation and elimination kinetics of sulfated CCK-8.

Main Methods:

Injection of radiolabeled inorganic sulfate ([35S]sulfate) into the rat cerebral cortex.
High-Performance Liquid Chromatography (HPLC) to measure radiolabeled CCK-8 formation and elimination.

Main Results:

The turnover rate of CCK-8 was calculated for the first time.

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CCK-8 exhibits a considerably slower turnover rate (half-life = 16 hours) compared to biogenic amines and amino acid neurotransmitters (half-lives < 4 hours).

Conclusions:

CCK-8 is a relatively stable neuropeptide in the brain.
The slow turnover of CCK-8 suggests distinct functional roles compared to faster-acting neurotransmitters.