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Phospholipase A2 activity modulates "ischemic effect" in pig skin.

O Nemoto, T Aoyagi, Y Miura

    The Journal of Investigative Dermatology
    |November 1, 1983
    PubMed
    Summary

    The skin

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    Area of Science:

    • Dermatology
    • Biochemistry

    Background:

    • The epidermis exhibits a rapid increase in cyclic AMP levels upon separation from in vivo conditions, known as the 'ischemic effect'.
    • The precise molecular mechanisms underlying this ischemic effect require further elucidation.

    Purpose of the Study:

    • To investigate the role of phospholipase A2 in the epidermal ischemic effect.
    • To explore the impact of melittin and mepacrine on cyclic AMP levels during skin ischemia.

    Main Methods:

    • Studied the effects of melittin (a phospholipase A2 stimulator) and mepacrine (a phospholipase A2 inhibitor) on isolated skin.
    • Measured cyclic AMP levels in the epidermis under ischemic conditions.

    Main Results:

    • Melittin treatment attenuated the rise in cyclic AMP levels during ischemia.
    • Mepacrine treatment potentiated the increase in cyclic AMP levels during ischemia.
    • These findings implicate phospholipase A2 activity in the ischemic response.

    Conclusions:

    • Phospholipase A2 activity appears to be a key factor in inducing the epidermal ischemic effect.
    • Membrane alterations mediated by phospholipase A2 may contribute to the observed changes in cyclic AMP levels.

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