Establishment of lymphoblastoid cell lines from Marek's disease primary tumors

Seven lymphoblastoid cell lines were developed from 15 Marek's disease primary lymphomas treated to remove adherent cells (46.7% success rate) and 1 cell line was developed from 15 untreated lymphomas (6.7% success rate). These cell lines are designated as MDCC-RP22 through MDCC-RP29. Tumor materials were from 151(5) x 7(1) chickens infected with either JM-111S clone or the GA-22 clone of Marek's disease virus. Factors contributing to the establishment of cell lines include, in addition to the removal of adherent cells at the initial stage of culture, the periodic adjustment and the concentration of viable cells, including the removal of dead cells. The cell lines bear T-cell surface markers and Marek's disease tumor associated surface antigen (MATSA). MDCC-RP22 and MDCC-RP23 are high virus producers.