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Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
09:03

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

Published on: October 16, 2017

Antigenic and structural studies on the transforming proteins of Rous sarcoma virus and Yamaguchi 73 avian sarcoma

E Erikson, R L Erikson

    Virology
    |October 15, 1983
    PubMed

    Insights

    Antisera against denatured pp60v-src revealed antigenic links between pp60v-src and P90gag-yes, a transforming protein from Class III avian sarcoma viruses. Peptide analysis confirmed structural similarities, highlighting shared characteristics in viral oncogene products.

    Area of Science:

    • Oncology
    • Virology
    • Molecular Biology

    Background:

    • pp60v-src is the transforming protein encoded by Rous sarcoma virus.
    • Avian sarcoma viruses share transforming gene products with potential antigenic relationships.

    Purpose of the Study:

    • To investigate the antigenic relationships between pp60v-src and transforming gene products of other avian sarcoma viruses.
    • To characterize the structure of P90gag-yes, the transforming protein of Class III avian sarcoma viruses.

    Main Methods:

    • Utilized a cross-reactive antiserum against denatured pp60v-src.
    • Performed tryptic phosphopeptide analysis on P90gag-yes.

    Main Results:

    • Demonstrated an antigenic relationship between pp60v-src and P90gag-yes.
    • Identified two phosphotyrosine-containing peptides and one phosphoserine-containing peptide in P90gag-yes.
    • Observed comigration of one P90gag-yes phosphotyrosine peptide with the pp60v-src phosphotyrosine peptide.

    Conclusions:

    • P90gag-yes, a Class III avian sarcoma virus transforming protein, is antigenically related to pp60v-src.
    • Structural similarities exist between pp60v-src and P90gag-yes, indicated by shared phosphopeptide analysis results.

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