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Chemical composition of human bone.

K J Quelch, R A Melick, P J Bingham

    Archives of Oral Biology
    |January 1, 1983
    PubMed
    Summary
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    Neonatal human bone has more glycoproteins and organic material than adult bone. Differences in sialic acid peaks among human, rabbit, and beef bone suggest species-specific variations in bone matrix composition.

    Area of Science:

    • Biochemistry
    • Bone Biology
    • Biomaterials Science

    Background:

    • Bone matrix comprises mineral and non-collagenous organic components.
    • Understanding bone matrix composition is crucial for bone health research.

    Purpose of the Study:

    • To analyze the mineral and non-collagenous organic components of human femoral cortex.
    • To compare neonatal and adult human bone matrix composition.
    • To investigate species-specific differences in bone matrix.

    Main Methods:

    • Powdering, demineralization with EDTA, and collagenase digestion.
    • Quantification of protein, hexose, sialic acid, and uronic acid.
    • Gel filtration (Sephadex G100) and ion-exchange chromatography (DEAE-cellulose).

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    Main Results:

    • Neonatal bone showed lower mineral/calcium and higher organic material/sialic acid than adult bone.
    • Gel filtration yielded four fractions; ion-exchange chromatography yielded six fractions.
    • Human bone matrix was more resistant to collagenase; sialic acid showed three peaks, suggesting proteoglycan binding.

    Conclusions:

    • Neonatal bone has increased glycoprotein content.
    • Human bone matrix composition differs from beef and rabbit bone, indicating species specificity.
    • Sialic acid distribution provides insights into bone matrix structure and species variations.