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Updated: Aug 12, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
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Pokeweed mitogen, Bordetella pertussis and breast milk cell factor induce preferentially the synthesis of different

M Puskás, F Antoni, F Péterfy

    Bollettino Dell'Istituto Sieroterapico Milanese
    |July 1, 1983
    PubMed

    Human tonsillar lymphocytes were cultured in the presence of different activators and [14C]-isoleucine. De novo synthesized, [14C]-labeled immunoglobulin was determined after separation of the different classes by immunoadsorbants carrying class-specific anti-human IgA, IgG, IgM, IgD or IgE. Pokeweed mitogen and whole killed Bordetella pertussis enhanced the synthesis and secretion of IgA, IgG and IgM. Maximum stimulation was found with pokeweed mitogen in IgM secretion (up to 5-fold), while Bordetella pertussis had the largest impact on IgA and IgG (4-5 fold increase). The human milk cell factor (demonstrated by Pittard and Bill., Cell. Immunol. 1979, 42, 437.) in the supernatant of cultured milk cells stimulated selectively the synthesis of IgA (4-fold).

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