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Related Experiment Videos

Mammalian small intestinal phytase (EC 3.1.3.8).

J R Cooper, H S Gowing

    The British Journal of Nutrition
    |November 1, 1983
    PubMed
    Summary

    Phytase enzyme concentration varies across small intestines in rodents. Its brush border localization in rats suggests a key role in metal ion absorption from phytate complexes.

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    Area of Science:

    • Biochemistry
    • Gastroenterology
    • Nutritional Science

    Background:

    • Phytase (EC 3.1.3.8) is crucial for phytate hydrolysis.
    • Phytate complexes can inhibit metal ion absorption.
    • Understanding phytase distribution is vital for nutrient bioavailability.

    Purpose of the Study:

    • To quantify phytase concentration in the small intestine of various species.
    • To determine the subcellular localization of phytase in rat small intestine.
    • To elucidate the role of phytase in metal ion absorption.

    Main Methods:

    • Enzyme assays to measure phytase activity.
    • Protein quantification for normalization.
    • Histochemical analysis for enzyme localization.

    Main Results:

    • Phytase levels ranged from 0.12 units/mg protein in rats to 0.03 units/mg protein in rabbits.
    • Phytase was localized to the brush border of the rat small intestine.
    • Species-specific variations in phytase concentration were observed.

    Conclusions:

    • Phytase levels and localization are significant factors in intestinal metal uptake.
    • Proximity of released metal ions to absorptive surfaces enhances absorption.
    • This mechanism may prevent metal insolubility due to competing reactions.

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