Voltage clamp analysis of ethanol effects on pacemaker currents of Aplysia neurons

The effect of 4% ethanol on pacemaker currents of Aplysia neurons was studied under voltage clamp. In normal seawater the n-shape in the I-V disappeared and outward current increased. Ion substitution and drug blocking experiments determined that leakage current and the slow inward calcium current were decreased and that the outward currents, IA and IK, were increased. This knowledge can be used to explain ethanol effects on spontaneous firing patterns.