Comparison of GABA analogues at the crayfish stretch receptor neurone

The conductance increase induced by GABA and structurally related compounds has been measured in voltage clamped stretch receptor neurones of crayfish. GABA induced only at 10(-3) M a rapid conductance increase. The response to lower concentrations between 10(-6) and 10(-4) M developed slowly (20-60 min). The postsynaptic conductance increase induced by repetitive application of the same GABA concentration was progressively enhanced in the speed and magnitude. In the presence of nipecotic acid or in Na+-free Ringer solutions, the response to all GABA concentrations was instantaneous and constant for each concentration. Muscimol between 10(-6) and 10(-3) M caused instantaneous dose-dependent conductance increases. These results suggest the presence of a saturable GABA uptake system limiting the access of bath applied GABA, but not of muscimol, to postsynaptic receptor sites.