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A method for coupling cytomegalovirus antigens to aldehyde-fixed erythrocytes for use in passive hemagglutination
A method for coupling cytomegalovirus (CMV) antigens to aldehyde-fixed erythrocytes for use in a passive hemagglutination assay for antibody to CMV is described. This method uses acid buffer instead of tannic acid for coupling CMV-complement fixation (CF) antigen to the stabilized cells. The coated cells are stable for at least 6 mth at -70 degrees C and for 5 days at 4 degrees C. This provides an inexpensive reagent having a sensitivity for anti-CMV detection which compares favorably with that of the CF test and enzyme immunoassay.
A method for coupling cytomegalovirus (CMV) antigens to aldehyde-fixed erythrocytes for use in a passive hemagglutination assay for antibody to CMV is described. This method uses acid buffer instead of tannic acid for coupling CMV-complement fixation (CF) antigen to the stabilized cells. The coated cells are stable for at least 6 mth at -70 degrees C and for 5 days at 4 degrees C. This provides an inexpensive reagent having a sensitivity for anti-CMV detection which compares favorably with that of the CF test and enzyme immunoassay.