Related Experiment Video

Updated: Aug 19, 2026

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
09:39

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Desensitization and cyclic AMP turnover in S49 cells

T J Goka, R Barber, B L Maxwell

    Journal of Cyclic Nucleotide and Protein Phosphorylation Research
    |January 1, 1983
    PubMed

    More Related Videos

    A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors12:27

    A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

    Published on: June 8, 2022

    Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ06:03

    Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ

    Published on: March 22, 2024

    Related Concept Videos

    Intracellular Signaling Cascades01:24

    Intracellular Signaling Cascades

    Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
    Positive Regulator Molecules02:39

    Positive Regulator Molecules

    Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
    Amplifying Signals via Second Messengers01:15

    Amplifying Signals via Second Messengers

    Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
    Amplifying Signals via Enzymatic Cascade01:22

    Amplifying Signals via Enzymatic Cascade

    When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
    Activation and Inactivation of G Proteins01:22

    Activation and Inactivation of G Proteins

    Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...
    GPCR Desensitization01:12

    GPCR Desensitization

    G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...