Comparison of 14C-labelled polyethylene glycol (PEG) with carrier PEG and 14C-PEG alone as a volume indicator in the human jejunum
Related Experiment Video
Updated: Jun 19, 2026

15:33
Microwave-assisted Functionalization of Poly(ethylene glycol) and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
Published on: October 30, 2013
Gut
|February 1, 1978
Abstract:
14C-labelled polyethylene glycol (PEG) (5 muCi/l) was used as a non-absorbable marker in human jejunum both with and without carrier PEG (5 g/l). Calculation of net water flux was virtually identical whether or not the carrier PEG was included in the perfusion solution. 14C-PEG alone is a satisfactory non-absorbable marker for perfusion studies in the human jejunum.
Related Concept Videos
Methods for Studying Drug Absorption: In vitro
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Methods for Studying Drug Absorption: In situ
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...

