Shedding patterns in swine of virulent and attenuated pseudorabies virus

Shedding patterns of 2 virulent (P-2208 and KC-152-D) and 1 attenuated (BUK) strains of pseudorabies virus (PRV) were determined in groups of intranasally inoculated feeder pigs. Clinical signs observed following inoculation with the P-2208 and KC-152-D strains included increase in rectal temperatures up to 42.2 C, anorexia, severe respiratory disturbance, and fatal CNS signs in 2 cases. Clinical signs in pigs inoculated with 7.2 X 10(7) median tissue culture infective dose (TCID50) of the BUK strain were limited to depression and a rise in rectal temperatures to 40.5 C for 3 to 4 days. Evaluation of the efficacy of the virus isolation method used showed that the presence on swabs of only 12.5 TCID50 of the P-2208 strain or 8.4 TCID50 of the BUK strain resulted in a 50% chance of virus recovery. Intranasal inoculations with 500 TCID50 of the P-2208 or KC-152-D strain did not result in synchronous infection of the whole group. Intranasal inoculations with 5,000 TCID50 of the KC-152-D strain or 50,000 TCID50 of the P-2208 strain resulted in continuous virus shedding in all pigs between postinoculation days (PID) 4 and 13 (KC-152-D strain) or 14 (P-2208 strain). Some of the pigs in these 2 groups further shed the P-2208 or KC-152-D strain in a continuous or discontinuous pattern up to PID 19 (P-2208 strain) or 20 (KC-152-D strain). The time of onset or the level of virus neutralizing antibody production in individual pigs was not found to have an influence on their shedding patterns.(ABSTRACT TRUNCATED AT 250 WORDS)