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Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
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Transport of potassium, amino acids, and glucose in cells transformed by Rous sarcoma virus

M J Weber, P K Evans, M A Johnson

    Federation Proceedings
    |January 1, 1984
    PubMed

    Insights

    Nutrient and ion transport rates in chicken cells change with growth and transformation. Malignant transformation alters amino acid and glucose transport, impacting cell metabolism.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Oncology

    Background:

    • Cellular nutrient and ion transport are crucial for growth and function.
    • Understanding transport regulation is key to deciphering cellular states like density inhibition and malignant transformation.

    Purpose of the Study:

    • To investigate how nutrient and ion transport rates change in chicken embryo fibroblasts under different conditions: exponential growth, density inhibition, and Rous sarcoma virus-induced transformation.
    • To elucidate the regulatory mechanisms of these transport systems.

    Main Methods:

    • Surveyed transport rates of potassium, alpha-aminoisobutyric acid (an amino acid analog), and glucose in chicken embryo fibroblasts.
    • Compared transport rates across exponentially growing, density-inhibited, and Rous sarcoma virus-transformed cells.

    Main Results:

    • All examined transport systems showed changes correlated with growth rate.
    • Ouabain-sensitive potassium transport declined in density-inhibited cells and increased with serum stimulation, regulated by transporter activity and number.
    • Alpha-aminoisobutyric acid transport decreased in density-inhibited cells and showed altered regulation in transformed cells.
    • Glucose transport exhibited both growth state-dependent and transformation-specific changes, with increased numbers of glucose transporters in transformed cells.
    • Elevated glucose transport preceded metabolic changes in glycolysis.

    Conclusions:

    • Nutrient and ion transport systems are dynamically regulated in response to cellular growth states and malignant transformation.
    • Changes in glucose transport, driven by increased transporter numbers, are essential for subsequent metabolic alterations in transformed cells.

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